The Structure of Yeast Nucleic Acid
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چکیده
Levene and Jacobs have formulated the structure of yeast nucleic acid as a tetranucleotide. The facts that led up to their formulation were: 1. The formation of four nucleosides on neutral or ammonia hydrolysis. 2. The formation of simpler nucleotides on hydrolysis with dilute mineral acids. 3. The presence of a phosphorus to nitrogen ratio which agreed quite well for t.he tetranucleotide theory. 4. The ratio of amino to total nitrogen in nucleic acid was in harmony with the ratio required by the four bases, guanine, adenine, uracil, and cytosine. The mode of linkage between the individual nucleotides was at that time not determined, and in the graphic formula representing the nucleic acid the linkage of the nucleotides was of a provisional character and arbitrary in nature. Following that, the studies of Levene and Jacobs on thymus nucleic acid led to methods which permitted the separation and the study of individual monoand dinucleotides composing the complex nucleic acids. Levene and Jacobs then returned to the study of yeast nucleic acid, applying the experience gained on the thymus nucleic acids. They then directed their attention to the nucleotides obtained by acid hydrolysis of the yeast nucleic acid. A large quantity of the material was prepared and transformed into the brucine salt. Other work, however, made a demand on their energies, and the work on the nucleotides was somewhat neglected. However, in course of the present academic year the work has been resumed, There were on hand 125.0 gm. of the brucine salts when the work was begun.
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